T4 DNA Ligase 40000U – High Efficiency Enzyme BC9K-005-0002

Description

Introduction

The T4 DNA Ligase 40000U is a premium grade molecular biology reagent engineered for seamless DNA fragment joining. Whether you are performing routine subcloning, library construction, or complex genetic assembly, this enzyme delivers the reliability and high-level performance necessary for successful laboratory research and biotechnology applications.

Key Features

– High concentration 40000U for optimized ligation kinetics.

– Catalyzes the formation of phosphodiester bonds between adjacent 5 phosphate and 3 hydroxyl termini.

– Compatible with both sticky cohesive and blunt-end DNA fragments.

– Rapid ligation protocol suitable for standard and fast reaction setups.

– Formulated for maximum stability during freeze-thaw cycles.

– Stringent quality control testing for endo and exonuclease contamination.

Benefits

This high-activity T4 DNA Ligase significantly reduces your troubleshooting time by providing robust ligation even under challenging experimental conditions. Its high concentration allows for smaller reaction volumes, saving you precious reagents and helping you maintain the purity of your genetic samples. By choosing our enzyme, you ensure higher transformation efficiency and a greater number of positive clones, allowing your research project to move forward faster and with greater confidence.

Usage / How to Use

To use the T4 DNA Ligase, combine your DNA fragments with the provided ligation buffer and the enzyme in a microcentrifuge tube. Incubate the mixture at the recommended temperature, typically 16 degrees Celsius for overnight ligation or room temperature for rapid protocols. After incubation, the reaction can be directly used for bacterial transformation or stored at minus 20 degrees Celsius for long-term stability. Always maintain the enzyme on ice during preparation to preserve its enzymatic activity.

Why Choose This Product

Our T4 DNA Ligase is manufactured under strict quality standards to ensure batch-to-batch consistency that you can rely on for your critical publications. We provide a product that minimizes background noise in your cloning projects, ensuring that you save time on screening colonies. With our focus on purity and performance, you get a premium reagent that supports the success of your laboratory work from start to finish.