Magrose 65 IDA-Ni Magnetic Beads for His-tag Protein Purification

Description

Introduction

The Magrose 65 IDA-Ni magnetic beads represent the gold standard for efficient His-tagged protein purification. These high-performance beads are specifically designed to simplify complex protein isolation tasks while maintaining the structural integrity and biological activity of your target samples.

Key Features

– Optimized for high-affinity binding of recombinant His-tagged proteins

– Fast magnetic separation speeds up washing and elution steps

– Highly cross-linked agarose base provides excellent mechanical stability

– Minimal non-specific binding ensures high-purity yield

– Compatible with both manual and automated liquid handling systems

– Consistent bead diameter for uniform performance across batches

Benefits

Using these magnetic beads significantly reduces the time required for protein purification compared to traditional column-based chromatography. By minimizing sample handling and providing a rapid magnetic recovery process, researchers can prevent protein degradation and increase overall laboratory productivity. The high binding capacity allows for the recovery of larger quantities of protein from smaller sample volumes, making it an cost-effective solution for both academic and industrial biotech applications.

Usage / How to Use

These beads are designed for use in common protein purification workflows. Simply add the magnetic beads to your clarified cell lysate and incubate to allow the His-tagged proteins to bind to the nickel-charged surface. Place the sample on a magnetic rack to pull down the beads, discard the supernatant, wash away impurities with your designated buffer, and finally elute your pure protein using an imidazole gradient. This process is highly reproducible and easy to scale up or down based on your experimental requirements.

Why Choose This Product

– Unmatched purity levels suitable for sensitive downstream applications like X-ray crystallography and NMR.

– Reliable performance that minimizes batch-to-batch variability in your research data.

– User-friendly protocols that save time and reduce technical errors in the lab.

– Robust chemical stability allowing for regeneration and reuse, providing excellent value for your budget.