RIPA Buffer II with EDTA and EGTA 2x Concentrate
Achieve superior protein extraction with our high-performance RIPA Buffer II. Designed for robust cell lysis, this 2x concentrate includes EDTA and EGTA to ensure maximum protease inhibition and sample integrity. Perfectly balanced at pH 7.4, it is the essential solution for Western blotting, immunoprecipitation, and downstream proteomic applications in your laboratory.
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Description
Introduction
The RIPA Buffer II with EDTA and EGTA is a high-strength lysis buffer formulated to effectively extract total proteins from a wide variety of cell types and tissues. By combining essential chelating agents with optimized detergent concentrations, this product provides a reliable foundation for consistent and reproducible molecular biology results.
Key Features
– Optimized 2x concentrate formulation for flexible experimental design.
– Includes EDTA and EGTA for potent inhibition of metalloproteases.
– Precisely adjusted to pH 7.4 to maintain physiological protein stability.
– Highly compatible with standard downstream applications like Western blot and ELISA.
– Designed to minimize background interference for cleaner membrane imaging.
– Stable formulation ensuring long shelf life and consistent performance across batches.
Benefits
This specialized buffer simplifies your workflow by eliminating the need for separate protease inhibitor cocktails in many standard protocols. The inclusion of EDTA and EGTA prevents the degradation of delicate protein targets, allowing you to recover high yields of functional proteins. By ensuring efficient cell membrane disruption, it helps you achieve sharper, clearer bands on your Western blots, significantly reducing the need for repeated experiments and saving your valuable research time.
Usage / How to Use
To use, dilute the 2x concentrate with ultrapure water to a 1x working solution. Add the buffer to your cell pellet or tissue sample on ice. Incubate for 15 to 30 minutes with gentle agitation to ensure thorough lysis. Centrifuge the mixture at high speed to pellet cell debris, then collect the supernatant containing your purified protein. The resulting lysate is ready for protein quantification or direct loading onto SDS-PAGE gels.
Why Choose This Product
– Quality assurance: Each batch is rigorously tested for consistent pH and chemical composition.
– Time efficiency: The 2x concentrated format allows for easy storage and quick dilution to your desired working volume.
– Scientific reliability: Trusted by researchers for providing clean, high-yield extractions across diverse mammalian cell lines.
– Cost-effective: Superior value without compromising on the purity or efficacy required for high-stakes research projects.
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