TMB Dihydrochloride Substrate for ELISA BC0514

Description

Introduction

TMB dihydrochloride is a premium-grade chromogenic substrate designed for use with horseradish peroxidase (HRP) in enzyme-linked immunosorbent assays. This reliable chemical agent is essential for clinical diagnostics and life science research, enabling accurate detection and quantification of biomolecules through clear, measurable colorimetric changes.

Key Features

– High-purity formulation for maximum sensitivity

– Fast reaction kinetics for efficient workflow

– Excellent stability for consistent assay performance

– Ideal for standard ELISA and blotting applications

– Compatible with common HRP conjugate systems

– Minimal background noise for clear signal detection

Benefits

Using this TMB dihydrochloride substrate allows researchers to achieve superior assay sensitivity, ensuring that even low-abundance targets are accurately detected. By reducing background interference, it streamlines data interpretation and increases confidence in experimental results. Its high stability reduces the risk of batch-to-batch variation, saving valuable time and reagents in demanding laboratory environments.

Usage / How to Use

This product is typically prepared as a working solution and added to microplate wells containing HRP-labeled conjugates. Upon addition, the TMB dihydrochloride undergoes oxidation to produce a distinct blue color. The reaction is commonly stopped by adding a sulfuric or phosphoric acid solution, which shifts the color to yellow for precise photometric measurement at 450 nm. It is best used in a controlled laboratory setting by trained personnel following standard immunoassay protocols.

Why Choose This Product

– Trusted quality ensures reproducibility across multiple experiments

– Optimized for clear and consistent optical density readings

– Trusted by laboratories for robust diagnostic and research applications

– Offered in multiple pack sizes to suit various throughput requirements